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KMID : 0043320070300070890
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Archives of Pharmacal Research
2007 Volume.30 No. 7 p.890 ~ p.897
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Determination of Bevantolol in Human Plasma by High Performance Liquid Chromatography Using Solid Phase Extraction Technique
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Trung Tran Quoc
Long Pham Hai
Al-Abd Ahmed M
Kuh Hyo-jeong
Lee Ho-Yoon
Hwang Sung-Joo
Kim Kyeong-Ho
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Abstract
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A method was developed and fully validated for the determination of bevantolol, an adrenergicreceptor blocker, in human plasma. Bevantolol and betaxolol as internal standard (I.S) were extracted from 1mL of human plasma by solid phase extraction technique using Sep-pak silica cartridge. Chromatographic separation was accomplished under isocratic conditions using a reverse-phase C8 analytical column and mixture of dibasic ammonium phosphate (pH 5.7; 50mM)-acetonitrile (75:25, v/v) as mobile phase, with a detection wavelength at 220 nm. The method was proved to be specific by testing six different human plasma sources. Linearity was established for the concentration ranges of 40-1600 ng/mL with correlation coefficent of 0.9995. The lower limit of quantification 40 ng/mL with precision of 10.9% as C.V%.
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KEYWORD
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Bevantolol, Betaxolol, HPLC, Solid phase extraction, Plasma
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